The flow cytometers Diaries
The flow cytometers Diaries
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Data-enriched characterization of cell populations. Morphological information from pictures can increase towards the richness of flow cytometry info. One example is, the determine shows an otherwise conventional apoptosis assay utilizing Annexin V and PI, incorporating mobile imaging to characterize cells in Every single populace to reveal morphologically distinctive attributes. These insights could not are actually gained from multiplex staining alone.
No matter whether you’re a novice or an authority, discover our resources to progress your investigation and skills in flow cytometry.
Combine acoustic focusing technological know-how with superior-parameter analysis for flexible spectral unmixing and conventional workflows.
Explore new insights into your experimental info by visualizing populations applying blended fluorescence and morphology information. Our application interface wizards permit downsampling, concatenation (merged files) and dimensionality resources which might be crafted to the computer software for an intuitive, streamlined information Investigation knowledge.
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In addition, it can be illuminating to see the quantity of doublets slip by means of standard singlet gates, and excellent to confirm the status of unforeseen double-good gatherings in my Assessment."
Pair your Attune flow cytometer with an autosampler for elevated workflow effectiveness, and add robotic integration for maximum throughput.
Our researchers will design panels to suit your needs as persistently you would like—free of charge, no product or service invest in required.
The Attune NxT cytometer is the trustworthy workhorse instrument your lab can depend upon for steady and trustworthy functionality. The Attune CytPix product includes a high-speed brightfield digicam and AI-driven picture analysis application, with recent upgrades such as device Studying equipment and an intuitive person interface, enabling Improved data precision, precision, and a lot quicker insights into sample biology.
Maybe most importantly, these activities consist of cells of Obviously different phenotypes which may lead to incorrect conclusions about double favourable occasions (particularly in exceptional populations).
Seem waves permit precise cell alignment, causing Excellent details excellent and ~10X quicker sample flow fees.
We Formerly demonstrated the strength of imaging Auto-T/Ramos mobile interactions. Permit’s check out just the populace of finest interest, the double positive events, to learn more. We can now use extended morphological parameters (listed here, circularity and skewness of depth) to even more examine the options of such populations and refine gating on these events, rising data click here robustness.
Developed-in dimensionality reduction tools greater resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to substantiate anticipated population resolution in lysed full human blood. User can perspective activities from Every single cluster for Visible confirmation of mobile phenotypes.
No clean/no lyse leukocyte Assessment with imaging. Cells had been obtained from 24-hour-old blood by dilution in 1 mM EDTA ( Specialist UX/UI permits people to train AI models for expanded applications. (A) The “Prepare Design” feature offers annotation resources and real-time design coaching accuracy.
At the heart of each and every Attune Flow Cytometer lies our groundbreaking acoustic focusing engineering, which makes use of seem waves to meticulously align cells throughout the flow mobile. This progressive method surpasses classic hydrodynamic techniques, leading to faster processing speeds, Extraordinary facts quality, and improved sample throughput.
The Attune Xenith cytometer is usually a high-parameter spectral analyzer, offering equally spectral unmixing and traditional flow abilities for comprehensive and multipurpose mobile Assessment.
The Attune CytPix Flow Cytometer enables end users to visualize samples in the course of acquisition, verify the accuracy of gating tactics, and help final results with responsible documentation for publication functions.
What seemed to be a homogeneous inhabitants is actually additional assorted—an insight that ought to be viewed as when interpreting benefits.